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queE [2019-07-31 13:09:39]
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queE [2019-07-31 13:09:39]

7-carboxy-7-deazaguanine (CDG) synthase, required for the synthesis of the modified ribonucleotide queuosine
Locus
BSU_13740
Isoelectric point
4.96
Molecular weight
26.97 kDa
Protein length
243 aa Sequence Blast
Gene length
732 bp Sequence Blast
Function
tRNA modification
Product
7-carboxy-7-deazaguanine (CDG) synthase
Essential
no
Synonyms
ykvL
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Translation → tRNA modification and maturation

    • Gene

    Coordinates
    1,440,542 → 1,441,273

    The protein

    Catalyzed reaction/ biological activity

  • radical-mediated ring rearrangement required to convert 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) into 7-carboxy-7-deazaguanine (CDG) PubMed

    • Protein family

  • Radical SAM superfamily (according to UniProt)

    • Cofactors

  • Fe-S cluster PubMed
  • S-adenosyl-L-methionine, Mg(2+) PubMed

    • Structure

  • 5TGS PubMed

    • Expression and Regulation

    Operons

    Genes
    queC-queD-queE-queF
    Description
    PubMed

    Regulatory mechanism

  • preQ1 riboswitch: antitermination, in the absence of queuosine PubMed, in preQ1 riboswitch

    • Regulation

  • repressed in the presence of queuosine (preQ1 riboswitch) PubMed
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    Biological materials

    Mutant

  • MGNA-A790 (ykvL::erm), available at the NBRP B. subtilis, Japan
  • BKE13740 (ΔqueE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GAAAATTTCTAATACAGGAA, downstream forward: _UP4_GTATAATAGAAAGGAAGATG
  • BKK13740 (ΔqueE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GAAAATTTCTAATACAGGAA, downstream forward: _UP4_GTATAATAGAAAGGAAGATG

    • References

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